Increase of urinary extracellular‐superoxide dismutase level correlated with cyclic adenosine monophosphate

FEBS Letters

Kakumu, Shinichi¹  Adachi, Tetsuo²  Hara, Hirokazu²  Yamada, Harutaka¹  Futenma, Arao¹ 

[1] First Department of Internal Medicine, Aichi Medical University, Nagakute, Aichi, Japan;Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu, Japan

关键词: Extracellular superoxide dismutase;    cAMP;    N-acetyl-β-D-glucosaminidase;    Parathyroid hormone;    Renal tubule;    EC-SOD;    extracellular superoxide dismutase;    Cu;    Zn-SOD;    copper- and zinc-containing SOD;    Mn-SOD;    manganese-containing SOD;    cAMP;    cyclic adenosine monophosphate;    dbcAMP;    N 6-O 2′-dibutyryl cAMP;    8BrcAMP;    8-bromo cAMP;    IBMX;    3-isobutyl-1-methylxanthine;    CRE;    creatinine;    NAG;    N-acetyl-β-D-glucosaminidase;    PTH;    parathyroid hormone;   

DOI  :  10.1016/S0014-5793(99)01185-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Extracellular superoxide dismutase (EC-SOD) is a secretory protein that is the major SOD isozyme in extracellular fluids. Plasma EC-SOD levels are distributed in two discrete groups with the rare group having an enzyme with glycine instead of arginine-213, which causes a 10-fold higher serum level. Within the common phenotype group, the urinary EC-SOD level was significantly correlated with the urinary excretion of N-acetyl-β-D-glucosaminidase (NAG), but not with serum EC-SOD. EC-SOD appears not to be leaked from the plasma by glomerular filtration, but rather to be secreted from the renal tubule or its surrounding tissues. The urinary EC-SOD level was also significantly correlated with the urinary cyclic adenosine monophosphate (cAMP) level. cAMP analogues and adenylate cyclase modulators significantly stimulated the expression of EC-SOD but not other SOD isozymes in cultured fibroblast cell lines. Moreover, injection of parathyroid hormone, in Ellsworth-Howard tests, increased urinary EC-SOD accompanied with the elevations of urinary cAMP and NAG. Together these observations suggest that factor(s) that stimulate the adenylate cyclase-cAMP system regulate the urinary EC-SOD level.

【 授权许可】

Unknown