Accumulation of mature mRNA in the nuclear fraction of mammalian cells

FEBS Letters

Gondran, Pierre¹  Dautry, François¹  Amiot, Franck¹  Weil, Dominique¹ 

[1] CNRS UPR 1983, Génétique Moléculaire et Intégration des Fonctions Cellulaires, Institut de Recherches sur le Cancer, B.P. 8, 94801 Villejuif, France

关键词: Nuclear mRNA metabolism;    Nuclear retention;    mRNA transport;    Nuclear matrix;   

DOI  :  10.1016/S0014-5793(99)01175-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Little is known about the nuclear mRNA content of mammalian cells. In this study, we analyzed by Northern blotting with a panel of probes the nuclear and cytoplasmic fractions derived from several rodent cell lines. For most of the genes under study, mature mRNAs could easily be detected in the nuclear fraction and accumulated to higher levels than the corresponding precursors. In addition, significant differences in the nucleo-cytoplasmic partition of mature mRNAs were observed between genes as well as between cell types (NIH 3T3, CTLL-2, D3-ES, PC-12), indicating that this nuclear accumulation of mRNA is regulated. Thus, while it is usually considered that splicing is the limiting step of pre-mRNA processing, these results point towards transport or nuclear retention of mRNA as a key determinant of nuclear mRNA metabolism.

【 授权许可】

Unknown