Inhibition of infection of incoming HIV‐1 virus by RNA‐cleaving DNA enzyme

FEBS Letters

Ling, Hong²  Hattori, Toshio²  Xu, Younong¹  Zhang, Xiaoyan² 

[1] Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University Kyoto, Japan;Division of Allergy and Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, 1-1, Seiryo-Machi, Aoba-ku, Sendai 980-857, Japan

关键词: DNA enzyme;    Inhibition of human immunodeficiency virus type 1 infection;    V3 loop;    Gene therapy;   

DOI  :  10.1016/S0014-5793(99)01149-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nine different DNA enzymes (DzV3-n, n=1–9) targeting the V3 loop region of HIV-1 HXB2 were synthesized. One of those, DzV3-9, efficiently cleaved the target in the conserved sequence in the RNA transcript in vitro. DzV3-9 was stable in the cells and inhibited replication of both NL432 and SF162 strains in U87 cells expressing CD4 and co-receptors. The inhibitory effect of DNAzyme on incoming HIV-1 was also demonstrated with pseudotype virions generated by NL432-based luciferase reporter genes. Thus, an efficient, stable DNAzyme against a functionally important region of HIV-1 was identified, and it may be useful for prevention of HIV-1 infection.

【 授权许可】

Unknown