FEBS Letters | |
Inhibition of infection of incoming HIV‐1 virus by RNA‐cleaving DNA enzyme | |
Ling, Hong2  Hattori, Toshio2  Xu, Younong1  Zhang, Xiaoyan2  | |
[1] Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University Kyoto, Japan;Division of Allergy and Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, 1-1, Seiryo-Machi, Aoba-ku, Sendai 980-857, Japan | |
关键词: DNA enzyme; Inhibition of human immunodeficiency virus type 1 infection; V3 loop; Gene therapy; | |
DOI : 10.1016/S0014-5793(99)01149-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Nine different DNA enzymes (DzV3-n, n=1–9) targeting the V3 loop region of HIV-1 HXB2 were synthesized. One of those, DzV3-9, efficiently cleaved the target in the conserved sequence in the RNA transcript in vitro. DzV3-9 was stable in the cells and inhibited replication of both NL432 and SF162 strains in U87 cells expressing CD4 and co-receptors. The inhibitory effect of DNAzyme on incoming HIV-1 was also demonstrated with pseudotype virions generated by NL432-based luciferase reporter genes. Thus, an efficient, stable DNAzyme against a functionally important region of HIV-1 was identified, and it may be useful for prevention of HIV-1 infection.
【 授权许可】
Unknown
【 预 览 】
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RO201912020308287ZK.pdf | 219KB | download |