Determination of carbohydrate‐deficient transferrin separated by lectin affinity chromatography for detecting chronic alcohol abuse

FEBS Letters

Ohkura, Takashi⁴  Yuasa, Isao⁵  Yoshikawa, Kiyoshi¹  Yamashita, Katsuko⁴  Shinzawa, Haruhide²  Umetsu, Kazuo¹  Maruyama, Katsuya³  Suzuki, Tsuneo¹ 

[1] Department of Forensic Medicine, Yamagata University School of Medicine, Yamagata 990-9585, Japan;Second Department of Internal Medicine, Yamagata University School of Medicine, Yamagata 990-9585, Japan;Department of Internal Medicine, National Institute on Alcoholism, Kurihama National Hospital, Yokosuka 239-0841, Japan;Department of Biochemistry, Sasaki Institute, Tokyo 101-0062, Japan;Department of Legal Medicine, Faculty of Medicine, Tottori University, Yonago 683-8503, Japan

关键词: Carbohydrate-deficient transferrin;    Chronic alcohol abuse;    Carbohydrate-deficient glycoprotein syndrome type 1A;    Lectin affinity chromatography;    CDT;    carbohydrate-deficient transferrin;    allo A;    Allomyrina dichotoma agglutinin;    TJA-I;    Trichosanthes japonica agglutinin I;    CAA;    chronic alcohol abuse;    Sia;    sialic acid;    Gal;    galactose;    GlcNAc;    N-acetylglucosamine;    CDGS 1A;    carbohydrate-deficient glycoprotein syndrome type 1A;    HCC;    hepatocellular carcinoma;    IEF;    isoelectric focusing;   

DOI  :  10.1016/S0014-5793(99)01137-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Carbohydrate-deficient transferrin (CDT) has been established as a valuable biological marker for detecting chronic alcohol abuse. To improve the diagnostic efficiency, we studied new CDT determination procedures involving the use of lectin affinity chromatography with Allomyrina dichotoma agglutinin (allo A) and Trichosanthes japonica agglutinin I (TJA-I) to isolate the CDT isoforms CDT-allo A and CDT-TJA, respectively. These procedures, based on detection of the CDT-allo A and CDT-TJA isoforms in sera, showed high sensitivity (100% and 98%, respectively) and high specificity (93% and 85%, respectively). These results demonstrate that the new procedures involving the use of lectin affinity chromatography are more useful for isolating markers in the CDT test than the conventional charge-based separation method.

【 授权许可】

Unknown