Gangliosides GM1 and GD1b are not polarized in mature hippocampal neurons

FEBS Letters

Burack, Michelle A²  Futerman, Anthony H¹  Banker, Gary²  Shogomori, Hidehiko¹ 

[1] Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel;Center for Research on Occupational and Environmental Toxicology, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97201-3098, USA

关键词: Ganglioside;    Cholera toxin;    Neuron;    Axon;    Dendrite;    Polarity;   

DOI  :  10.1016/S0014-5793(99)01146-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Analysis of the binding of cholera toxin to ganglioside GM1 in both living and fixed neurons, and comparison with the distribution of defined axonal and dendritic proteins, demonstrates that ganglioside GM1 is distributed in a non-polarized manner over the axonal and dendritic plasma membranes of mature, cultured hippocampal neurons. Likewise, ganglioside GD1b is also distributed in a non-polarized manner. These results suggest that a recent report [Ledesma, M.D. et al. EMBO J. 18 (1999) 1761–1771] proposing that ganglioside GM1 is highly enriched on the axonal versus dendritic membrane of hippocampal neurons may need to be re-evaluated.

【 授权许可】

Unknown