The delta subunit of rod specific cyclic GMP phosphodiesterase, PDE δ, interacts with the Arf‐like protein Arl3 in a GTP specific manner

FEBS Letters

Becker, Jörg¹  Hanzal-Bayer, Michael¹  Linari, Marco¹ 

[1] Max-Planck-Institut für Molekulare Physiologie, Abteilung Strukturelle Biologie, Otto-Hahn-Straße 11, 44227 Dortmund, Germany

关键词: Phosphodiesterase δ;    Retinitis pigmentosa;    Spectroscopy;   

DOI  :  10.1016/S0014-5793(99)01117-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recently, we have shown that the δ subunit of the cGMP phosphodiesterase (PDE δ) interacts with the retinitis pigmentosa guanine regulator (RPGR). Here, using the two-hybrid system, we identify a member of the Arf-like protein family of Ras-related GTP-binding proteins, Arl3, that interacts with PDE δ. The interaction was verified by fluorescence spectroscopy and co-immunoprecipitation. Arl3 features an unusually low affinity for guanine nucleotides, with a K _D of 24 nM for GDP and 48 μM for GTP. Fluorescence spectroscopy shows that PDE δ binds and specifically stabilizes the GTP-bound form of Arl3 by strongly decreasing the dissociation rate of GTP. Thus, PDE δ is an effector of Arl3 and could provide a novel nucleotide exchange mechanism by which PDE δ stabilizes Arl3 in its active GTP-bound form.

【 授权许可】

Unknown