Distinct roles of profilin in cell morphological changes: microspikes, membrane ruffles, stress fibers, and cytokinesis

FEBS Letters

Takenawa, Tadaomi¹  Suetsugu, Shiro¹  Miki, Hiroaki¹ 

[1] Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo, Japan

关键词: Actin;    Profilin;    Small G protein;    Cytokinesis;    Cell morphology;   

DOI  :  10.1016/S0014-5793(99)01086-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we report the functional importance of profilin in various actin-mediated morphological changes using H119E mutant profilin I, which is deficient only in actin binding. In the case of actin-protrusive structures from the plasma membrane, H119E-profilin was shown to suppress the formation of Cdc42-induced actin microspikes and Rac-induced membrane ruffles. Conversely, Rho-induced stress fiber formation seemed to occur independently of H119E-profilin introduction. Furthermore, H119E-profilin blocked cleavage furrow ingression and subsequent adhesion to the substratum during cell division, a process in which actin plays indispensable roles.

【 授权许可】

Unknown