Alternative exon 3 splicing of the human major protein zero gene in white blood cells and peripheral nerve tissue

FEBS Letters

Boutrand, L.³  Kopp, N.²  Latour, P.¹  Besançon, R.³  Prost, A.L.³  Vandenberghe, A.³  Konecny, L.³  Petiot, P.²  Chamba, G.³  Mularoni, A.³ 

[1]Unité de Neurogénétique, Laboratoire de Biochimie, HCL, Hôpital de l'Antiquaille, F-69005 Lyon, France

[2]Hôpital Neurologique, 59 boulevard Pinel, F-69003 Lyon, France

[3]Laboratoire de Génétique Moléculaire Humaine, Faculté de Pharmacie, 8 avenue Rockefeller, F-69008 Lyon, France

关键词: Major protein zero;    Myelin;    Splicing;    Premature termination codon;   

DOI  :  10.1016/S0014-5793(99)01069-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The major protein zero (MPZ) is involved in peripheral myelin folding. Using nested reverse transcription-PCR, we amplified several fragments of MPZ mRNAs in white blood cells and in peripheral nerve tissue. Cloning of PCR products revealed the existence of three alternative splicing patterns: one resulted in the complete loss of exon 3 and two others induced partial skipping of the exon 3 sequence. All three alternative splicing mechanisms produced a frame-shift and created an identical premature stop codon in exon 4. We conclude that the existence of these MPZ RNA transcript variants may be the result of deliberate splicing decisions and may have functional implications in the cell.

【 授权许可】

Unknown