Association of the rat heterogeneous nuclear RNA‐ribonucleoprotein F with TATA‐binding protein

FEBS Letters

Yoshida, Tatsushi¹  Tamura, Taka-aki²  Makino, Yasutaka¹ 

[1]Department of Biology, Faculty of Science, Chiba University, Chiba, Japan

[2]CREST Japan Science and Technology Corporation, 1-33 Yayoicho, Inage-ku, Chiba 263-8522, Japan

关键词: Heterogeneous nuclear ribonucleoprotein F;    Splicing factor;    TATA-binding protein;    Transcription factor;    hnRNP-F;    heterogeneous nuclear ribonucleoprotein F;    TBP;    TATA-binding protein;    RNAP II;    RNA polymerase II;    CTD;    carboxy-terminal domain;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    PIC;    pre-initiation complex;    GTF;    general transcription factor;    HXmTBP;    histidine-tagged mouse TBP;    CPSF;    cleavage-polyadenylation specificity factor;    TIP;    TBP-interacting protein;    GST;    glutathione S-transferase;    PMSF;    phenylmethylsulfonyl fluoride;   

DOI  :  10.1016/S0014-5793(99)01048-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Heterogeneous nuclear ribonucleoprotein F (hnRNP-F) has been shown to be a pre-mRNA splicing factor. Recent studies have uncovered the coordination of synthesis of pre-mRNA and its processing, including post-transcriptional modification and splicing. Here, we present evidence for an association between a splicing factor, hnRNP-F, and TATA-binding protein (TBP), which is an essential factor needed for transcription initiation. An affinity detection experiment revealed hnRNP-F in the preparation of TBP-interacting proteins. HnRNP-F was associated with TBP in nuclear extracts and was capable of direct binding to TBP in vitro. These results suggest that hnRNP-F is associated with TBP in the cell. HnRNP-F was observed in abundance in the thymus, spleen and testis, and its distribution pattern was similar to that of TBP, implying a functional coordination of transcription and splicing. We assume that the splicing machinery is associated with the transcription apparatus as a prerequisite prior to transcriptional elongation.

【 授权许可】

Unknown