FEBS Letters | |
Receptor‐mediated activation of phospholipase D by sphingosine 1‐phosphate in skeletal muscle C2C12 cells | |
Meacci, Elisabetta1  Farnararo, Marta1  Bruni, Paola1  Donati, Chiara1  Vasta, Valeria1  | |
[1] Dipartimento di Scienze Biochimiche, University of Florence, Viale G.B. Morgagni 50, 50134 Firenze, Italy | |
关键词: Phospholipase D; Sphingosine 1-phosphate; Protein kinase C; C2C12 myoblast; Edg receptor; | |
DOI : 10.1016/S0014-5793(99)01033-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The present study showed that sphingosine 1-phosphate (SPP) induced rapid stimulation of phospholipase D (PLD) in skeletal muscle C2C12 cells. The effect was receptor-mediated since it was fully inhibited by pertussis toxin. All known SPP-specific receptors, Edg-1, Edg-3 and AGR16/H218, resulted to be expressed in C2C12 myoblasts, although at a different extent. SPP-induced PLD activation did not involve membrane translocation of PLD1 or PLD2 and appeared to be fully dependent on protein kinase C (PKC) catalytic activity. SPP increased membrane association of PKCα, PKCδ and PKCλ, however, only PKCα and PKCδ played a role in PLD activation since low concentrations of GF109203X and rottlerin, a selective inhibitor of PKCδ, prevented PLD stimulation.
【 授权许可】
Unknown
【 预 览 】
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