Receptor‐mediated activation of phospholipase D by sphingosine 1‐phosphate in skeletal muscle C2C12 cells

FEBS Letters

Meacci, Elisabetta¹  Farnararo, Marta¹  Bruni, Paola¹  Donati, Chiara¹  Vasta, Valeria¹ 

[1] Dipartimento di Scienze Biochimiche, University of Florence, Viale G.B. Morgagni 50, 50134 Firenze, Italy

关键词: Phospholipase D;    Sphingosine 1-phosphate;    Protein kinase C;    C2C12 myoblast;    Edg receptor;   

DOI  :  10.1016/S0014-5793(99)01033-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The present study showed that sphingosine 1-phosphate (SPP) induced rapid stimulation of phospholipase D (PLD) in skeletal muscle C2C12 cells. The effect was receptor-mediated since it was fully inhibited by pertussis toxin. All known SPP-specific receptors, Edg-1, Edg-3 and AGR16/H218, resulted to be expressed in C2C12 myoblasts, although at a different extent. SPP-induced PLD activation did not involve membrane translocation of PLD1 or PLD2 and appeared to be fully dependent on protein kinase C (PKC) catalytic activity. SPP increased membrane association of PKCα, PKCδ and PKCλ, however, only PKCα and PKCδ played a role in PLD activation since low concentrations of GF109203X and rottlerin, a selective inhibitor of PKCδ, prevented PLD stimulation.

【 授权许可】

Unknown