Distribution and quantification of immunoreactive orexin A in rat tissues

FEBS Letters

Opacka-Juffry, Jolanta¹  Bloom, Stephen R.²  Ghatei, Mohammad A.²  Taheri, Shahrad²  Mahmoodi, Mehdi² 

[1] MRC cyclotron unit, The Hammersmith Hospital, Du Cane Road, London W12 0NN, UK;Endocrine Unit, Division of Investigative Sciences, Imperial College School of Medicine, The Hammersmith Hospital, Du Cane Road, London W12 0NN, UK

关键词: Orexin A;    Hypothalamus;    Central nervous system;   

DOI  :  10.1016/S0014-5793(99)01030-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A sensitive and specific radioimmunoassay for orexin A was developed. Orexin A immunoreactivity was found to be confined to the central nervous system (CNS) with the highest concentrations in the hypothalamus, inferior and superior colliculi and brainstem. Within the hypothalamus, the highest levels were found in the lateral and posterior hypothalamus. These regions had a greater orexin A content in females compared to males. The orexin A content of hypothalamic regions did not change with fasting and no difference was noted in hypothalami of rats fed a high fat diet. The hypothalamic orexin A content was not different in obese Zucker rats compared to lean controls. Thus, orexin A has a wide distribution in the CNS, but appetite regulation may not be its main function.

【 授权许可】

Unknown