Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase

FEBS Letters

Toivari, Mervi H¹  Penttilä, Merja¹  Richard, Peter¹ 

[1] VTT Biotechnology and Food Research, P.O. Box 1500, 02044 VTT, Finland

关键词: Xylitol dehydrogenase;    Xylose metabolism;    D-Xylulose;    Enzyme kinetics;    Saccharomyces cerevisiae;   

DOI  :  10.1016/S0014-5793(99)01016-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S. cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH. The following Michaelis constants were estimated: D-xylulose, 1.1 mM; NADH, 240 μM (at pH 7.0); xylitol, 25 mM; NAD, 100 μM (at pH 9.0). Xylitol dehydrogenase activity with the same kinetic properties can also be induced by xylose in wild type S. cerevisiae cells.

【 授权许可】

Unknown