ADP‐ribosylation factor (ARF)‐like 4, 6, and 7 represent a subgroup of the ARF family characterized by rapid nucleotide exchange and a nuclear localization signal

FEBS Letters

Weber, Yvonne¹  Schürmann, Annette¹  Schilf, Christiane¹  Jacobs, Stephan¹  Joost, Hans-Georg¹  Koling, Susanne¹  Fliegert, Frank¹ 

[1] Institut für Pharmakologie und Toxikologie, Medizinische Fakultät der RWTH Aachen, Wendlingweg 2, D-52057 Aachen, Germany

关键词: ADP-ribosylation factor;    GTPase;    Ras-related;    GTP binding;   

DOI  :  10.1016/S0014-5793(99)00759-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The novel ARF-like GTPase ARL7 is a close relative of ARL4 and ARL6 (71% and 59% identical amino acids). A striking characteristic of these GTPases is their basic C-terminus which, when fused to the C-terminus of green fluorescent protein (GFP), targets the constructs to the nucleus of transfected COS-7 cells. Full length ARL4 was detected in both nuclear and extranuclear compartments, whereas a construct of ARL4 lacking its C-terminus was excluded from the nucleus. Nucleotide exchange rates of recombinant ARL4, ARL6 and ARL7 were similar and appeared considerably higher than those of other members of the ARF family (ARF1, ARP). It is concluded that ARL4, ARL6 and ARL7 form a subgroup within the ARF family with similar, possibly nuclear, function.

【 授权许可】

Unknown