Involvement of Sp1 in basal and retinoic acid induced transcription of the human tissue‐type plasminogen activator gene

FEBS Letters

De Vriese, Astrid¹  Merchiers, Pascal¹  Bulens, Frank¹  Belayew, Alexandra¹  Collen, Désiré¹ 

[1] Center for Molecular and Vascular Biology, University of Leuven, Herestraat 49, B-3000 Leuven, Belgium

关键词: Tissue-type plasminogen activator;    Promoter-enhancer;    Sp1;    Retinoic acid receptor;   

DOI  :  10.1016/S0014-5793(99)00942-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transcription of the human tissue-type plasminogen activator (t-PA) gene is regulated by a multi-hormonal responsive enhancer at −7 kb. Transient co-transfections of Drosophila SL2 and human HT1080 fibrosarcoma cells with t-PA reporter constructs showed that Sp1 and Sp3 activate the t-PA promoter. Moreover Sp1 (but not Sp3) binding to the promoter is involved in induction by retinoic acid (RA), a response mediated through the enhancer. The role of Sp1 is specific, since mutation of the CRE element in the promoter did not affect response to RA. In contrast, the glucocorticoid induction mediated by the enhancer is independent of these Sp1 and CRE elements.

【 授权许可】

Unknown