In vitro production of cytokines is influenced by sulfatide and its precursor galactosylceramide

FEBS Letters

Bendtzen, Klaus³  Fredman, Pam¹  Buschard, Karsten²  Bovin, Lone Frier³  Månsson, Jan-Eric¹ 

[1] Institute of Clinical Neuroscience, Section of Experimental Neuroscience, University of Göteborg, Mölndal, Sweden;Bartholin Institute, H:S Kommunehospitalet, Copenhagen, Denmark;Institute for Inflammation Research, IIR 7521, H:S Rigshospitalet National University Hospital, Tagensvej 20, DK-2200 Copenhagen, Denmark

关键词: Sulfatide;    Galactosylceramide;    Interleukin;    Tumor necrosis factor;    Cytokine mRNA;    Gene expression;   

DOI  :  10.1016/S0014-5793(99)00908-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Effects of sulfatide and its precursor galactosylceramide (gal-cer) on the kinetics of production of cytokines were studied. In human mononuclear leucocytes, gal-cer but not sulfatide induced significantly increased amounts of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF) mRNA. In phytohemagglutinin-stimulated cultures, gal-cer increased the levels of IL-1β and IL-6 mRNA and secreted IL-1β and IL-6, while sulfatide decreased the amounts of IL-6 mRNA and secreted IL-6. Gal-cer also increased TNF secretion. In lipopolysaccharide-stimulated cells, sulfatide but not gal-cer decreased the secretion of IL-1β and IL-10, a potent suppressor of production of many cytokines. Thus, sulfatide and gal-cer affect cytokine production differently, most likely at the level of gene expression. This may have implications in diseases where inflammatory cytokines play a pathogenic role.

【 授权许可】

Unknown