Activation and expression of ERK, JNK, and p38 MAP‐kinases in isolated islets of Langerhans: implications for cultured islet survival

FEBS Letters

Cavanagh, Thomas J.²  Hering, Bernhard³  Maysinger, Dusica⁴  Wang, Rennian⁵  Aikin, Reid⁴  Paraskevas, Steven⁵  Lakey, Jonathan R.T.¹  Rosenberg, Lawrence⁵ 

[1] Medical Surgical Research Institute, University of Alberta, Edmonton, Alta., Canada;Roche Molecular Biochemicals, Indianapolis, IN, USA;Department of Surgery, University of Minnesota, Minneapolis, MN, USA;Department of Pharmacology, McGill University and The Montreal General Hospital, Montreal, Que., Canada;Department of Surgery, The Montreal General Hospital, Montreal, Que., Canada

关键词: Islets of Langerhans;    Mitogen activated protein kinase;    Extracellular signal regulated kinase;    JNK;    p38;    Apoptosis;   

DOI  :  10.1016/S0014-5793(99)00882-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Isolation and purification of islet cells exposes them to ischemic, osmotic and mechanical stresses. The objective of this study was to determine the roles of the MAP-kinases in islets immediately following isolation. During the first 48 h, activity of JNK1 and JNK2 declined markedly. Activity of p38 increased steadily with time in culture while extracellular signal regulated kinase (ERK) activity declined dramatically within 24 h post-isolation. High p38 activation relative to ERK activation immediately following isolation correlated with a decrease in islet survival after 36 h in culture. Absence and/or transiency of ERK signaling in conjunction with sustained activation of p38 pathway could be an important regulator of cell death in islets during and following their isolation by commonly employed procedures.

【 授权许可】

Unknown