Influence of polyamines on DNA binding of heat shock and activator protein 1 transcription factors induced by heat shock

FEBS Letters

Bernelli-Zazzera, Aldo¹  Dansi, Paola¹  Tacchini, Lorenza¹  Desiderio, Maria Alfonsina¹ 

[1]Istituto di Patologia Generale dell'Università degli Studi di Milano, Centro di Studio sulla Patologia Cellulare del CNR, via Mangiagalli 31, 20133 Milan, Italy

关键词: Heat shock;    Polyamine;    Transcription factor;    hsp 70;    heat shock protein 70;    HSF;    heat shock factor;    AP-1;    activator protein 1;    STAT;    signal transducer and activator of transcription;    DFMO;    α-difluoromethylornithine;    ODC;    ornithine decarboxylase;    AdoMetDC;    S-adenosylmethionine decarboxylase;    EMSA;    electrophoresis mobility shift assay;    HSE;    heat shock element;    TRE;    TPA responsive element;    SIE;    sis inducible elements;    oct-1;    octamer-1;   

DOI  :  10.1016/S0014-5793(99)00873-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyamine depletion, obtained in FAO cells with specific inhibitors of biosynthetic enzymes, prevents or decreases the accumulation of hsp 70 mRNA following heat shock [Desiderio et al., Hepatology 24 (1996) 150–156]. The present study shows that under conditions of spermidine depletion caused by α-difluoromethylornithine, the DNA binding capacity of the transcription factor HSF induced by heat shock undergoes a severe and prompt deactivation. Replenishment of the spermidine pool before heat shock re-establishes the DNA binding activity of HSF and the inducibility of hsp 70 mRNA. Similar to HSF, but with a different time-course, the DNA binding of the transcription factor AP-1 activated by heat shock is also impaired in spermidine-depleted cells and reversed by exogenous spermidine. STAT3 provides an example of a transcription factor slightly activated by heat shock but insensitive to polyamine decrease.

【 授权许可】

Unknown