Activation of PPARγ inhibits cell growth and induces apoptosis in human gastric cancer cells

FEBS Letters

Kohgo, Yutaka¹  Fujimoto, Yoshinori¹  Okumura, Toshikatsu¹  Takahashi, Nobuhiko¹  Motomura, Wataru¹  Kawabata, Isao¹ 

[1] Third Department of Internal Medicine and Department of Laboratory Medicine, Asahikawa Medical College, Asahikawa 078-8510, Japan

关键词: Peroxisome proliferator-activated receptor γ;    Growth inhibition;    Apoptosis;    Gastric cancer;    PPARγ;    peroxisome proliferator-activated receptor γ;    PPRE;    peroxisome proliferator response element;   

DOI  :  10.1016/S0014-5793(99)00871-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the expression of peroxisome proliferator-activated receptor γ (PPARγ) and the role of PPARγ in cell growth in human gastric cancer cells. Reverse transcription-polymerase chain reaction, Northern blot and Western blot analyses showed that a human gastric cancer cell line, MKN45, expressed PPARγ mRNA and protein. Luciferase assay in MKN45 cells showed that troglitazone, a selective ligand for PPARγ, transactivated the transcription of a peroxisome proliferator response element-driven promoter. Troglitazone or pioglitazone, selective ligands for PPARγ, inhibited the growth of MKN45 cells in a dose-dependent manner. Co-incubation of MKN45 cells with troglitazone induced DNA ladder formation. These results suggest that human gastric cancer cells express PPARγ and that activation of PPARγ inhibits cell growth and induces apoptosis in gastric cancer cells.

【 授权许可】

Unknown