Establishment of an embryonic stem (ES) cell line derived from a non‐obese diabetic (NOD) mouse: in vivo differentiation into lymphocytes and potential for germ line transmission

FEBS Letters

Niho, Yoshiyuki¹  Sakai, Yumiko²  Nagafuchi, Seiho¹  Kitamura, Daisuke⁴  Tsukiyama, Tadasuke¹  Kondo, Shiori¹  Watanabe, Takeshi³  Katsuta, Hitoshi¹  Akashi, Tomoyuki¹  Kogawa, Kazuhiko¹ 

[1] First Department of Internal Medicine, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan;Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan;Department of Molecular Immunology, Kyushu University, Fukuoka 812-8582, Japan;Division of Molecular Biology, Research Institute for Biological Sciences, Science University of Tokyo, Noda, Chiba 278, Japan

关键词: Embryonic stem cell;    Non-obese diabetic mouse;    Germ line transmission;    Diabetes;    Insulitis;   

DOI  :  10.1016/S0014-5793(99)00801-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A_β ^g7 haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established.

【 授权许可】

Unknown