Detection of putative Zn(II) binding sites within Escherichia coli RNA polymerase: inconsistency between sequence‐based prediction and 65Zn blotting

FEBS Letters

Sujatha, Sitaraman¹  Chatterji, Dipankar¹ 

[1] Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007 (A.P.), India

关键词: Zn(II) site;    Zn(II) finger;    65Zn blotting;    Atomic absorption;    Subunit location;   

DOI  :  10.1016/S0014-5793(99)00786-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The availability of repeating ‘Cys’ and/or ‘His’ units in a particular order prompts the prediction of Zn(II) finger motifs in a protein. Escherichia coli RNA polymerase has two tightly bound Zn(II) per molecule of the enzyme as detected by atomic absorption spectroscopy. One Zn(II) was identified to be at the β subunit, whereas the other putative Zn(II) binding site has recently been predicted to be at the N-terminal half of the β′ subunit, from primary sequence analysis. We show here that the β′ subunit has no ability to bind ⁶⁵Zn(II). On the other hand, the N-terminal domain of the α subunit has strong Zn(II) binding ability with no obvious functional implications.

【 授权许可】

Unknown