The effect of 17β‐estradiol‐DNA adducts on the replication of exon # 5 of the human suppressor gene p53

FEBS Letters

Wang, Mian-Ying²  Zheng, Wei-Yun²  Miller, James¹  Bender, Wanda²  Yu, Fu-Li²  Cheerva, Alexandra³ 

[1] Department of Obstetrics and Gynecology, University of Illinois College of Medicine at Rockford, Rockford, IL 61107, USA;Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL 61107, USA;Department of Pediatrics, University of Illinois College of Medicine at Rockford, Rockford, IL 61107, USA

关键词: Exon # 5 of p53;    17β-estradiol;    Dimethyldioxirane;    DNA adduct;    DNA replication;   

DOI  :  10.1016/S0014-5793(99)00769-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a PCR technique, exon # 5 of the human tumor suppressor gene p53 was amplified and ligated into the pCRII vector and transformed into Escherichia coli INVαF’ competent cells. The cloned exon # 5 was 184 bp long. Evidence is presented to show that after dimethyldioxirane epoxidation, 17β-estradiol was able to form 17β-estradiol-DNA adducts and to strongly inhibit the replication of the cloned exon # 5 producing smaller sizes of DNA fragments and introducing errors of incorporation at the 3′-end of the terminating DNAs. The errors occurred mainly at the clusters of the complementary ‘G’ and ‘A’ bases on the template strand DNA, presumably, the major sites where the 17β-estradiol-DNA adducts were formed.

【 授权许可】

Unknown