Plant cell growth and differentiation may involve GAP regulation of Rac activity

FEBS Letters

Pødenphant, Lone¹  Borg, Søren¹  Jensen, Trine Juul¹  Poulsen, Carsten¹ 

[1] Laboratory of Gene Expression, Department of Structural and Molecular Biology, University of Aarhus, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark

关键词: Two-hybrid screening;    Fusion protein expression;    GTPase activating protein;    Cdc42/Rac interactive binding element;    Root nodule development;    Lotus japonicus;    SMG;    small GTP binding;    GAP;    GTPase activating protein;    CRIB;    cdc42/Rac interactive binding;    MBP;    maltose binding protein;    GST;    glutathione S-transferase;    GppNHp;    guanylyl 5′-imidodiphosphate;    PBS;    phosphate buffered saline;   

DOI  :  10.1016/S0014-5793(99)00750-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two Rac GTPase cDNAs, LjRac1 and LjRac2, were identified in the legume Lotus japonicus. Two-hybrid screening with dominant-constitutive mutations in the two Rac GTPases target three plant cDNAs, LjRacGAP1, LjRacGAP2 and LjRacGAP3, that encode putative GTPase activating proteins of Rho-GTPase subfamily members. Employing Rac antiserum, purified recombinant LjRac GTPases and recombinant LjRacGAP1, for ligand overlay assays, in vitro GAP affinity assays and GTPase activation, we confirmed that eukaryote Rac/RacGAP interplay is conserved in plants. In this investigation we have developed some tools that can be used to characterize the role of enhanced LjRac2 expression in developing root nodules.

【 授权许可】

Unknown