Analysis of the in vivo interaction between a basic repressor and an acidic activator

FEBS Letters

Lehming, Norbert¹  Wellhausen, Anne¹ 

[1] Max-Delbrück-Laboratorium in der Max-Planck-Gesellschaft, Carl-von-Linné-Weg 10, 50829 Köln, Germany

关键词: Repression;    SSB24;    GCN4;    Split ubiquitin assay;   

DOI  :  10.1016/S0014-5793(99)00718-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The artificial basic repressor SSB24 represses transcription of a reporter construct activated by GCN4. We show that the positively charged SSB24 and the negatively charged acidic activator GCN4 interact in vitro and in vivo. However, deleting the interaction domain from the GCN4 activator does not result in loss of repression by SSB24. Similarly, transcription activated by the holoenzyme component SRB2 is repressed, although SSB24 and SRB2 do not interact. Repression by SSB24 therefore does not depend on the observed protein-protein interaction between SSB24 and GCN4.

【 授权许可】

Unknown