Protein kinase C‐θ is specifically activated in murine erythroleukaemia cells during mitosis

FEBS Letters

Pontremoli, Sandro¹  Passalacqua, Mario¹  Patrone, Mauro¹  Sparatore, Bianca¹  Melloni, Edon¹  Pedrazzi, Marco¹ 

[1] Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV, 1-16132 Genoa, Italy

关键词: Protein kinase C-θ;    Protein kinase C activation;    Murine erythroleukaemia cell cycle;    Enzymology;    PKC;    protein kinase C;    MEL;    murine erythroleukaemia;    HMBA;    hexamethylenebisacetamide;    PMA;    phorbol-12-myristate-13-acetate;    PS;    phosphatidylserine;   

DOI  :  10.1016/S0014-5793(99)00729-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein kinase C-θ is a member of the n-protein kinase C subfamily that in mitotic cells translocates to centrosomes and kinetochores. Although this kinase is expressed in comparable amounts in murine erythroleukaemia cells during the interphase or metaphase, when localized in the mitotic structures, it selectively phosphorylates a 66 kDa protein, also associated to chromosomes. Moreover, protein kinase C-θ immunoprecipitated from cells at the metaphase results four times more active in the absence of lipid cofactors as compared with the kinase obtained from cells in the interphase. This activation is accomplished by interaction of protein kinase C-θ with a protein factor which also promotes an increased auto-phosphorylation of the kinase. These findings indicate that in the mitotic phase of the cell cycle, protein kinase C-θ recognizes a protein factor which operates as a positive modulator of the kinase activity in the absence lipids.

【 授权许可】

Unknown