FEBS Letters | |
Exploiting retrograde transport of Shiga‐like toxin 1 for the delivery of exogenous antigens into the MHC class I presentation pathway | |
Dunbar, P.Rod2  Teisserenc, Hélène T.2  Cerundolo, Vincenzo2  Noakes, Karen L.1  Roberts, Lynne M.1  Lord, J.Michael1  | |
[1] Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK;Molecular Immunology Group, Institute of Molecular Medicine, Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, UK | |
关键词: Influenza Matrix protein; Shiga-like toxin A chain; Endoplasmic reticulum; BFA; brefeldin A; LF; anthrax toxin lethal factor; CTL; cytotoxic T lymphocytes; DT; diphtheria toxin; ER; endoplasmic reticulum; IFN-γ; interferon gamma; MHC; major histocompatibility complex; PE; Pseudomonas exotoxin A; SLT; Shiga-like toxin 1; | |
DOI : 10.1016/S0014-5793(99)00609-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Shiga-like toxin 1 (SLT) from Escherichia coli 0157:H7 enters mammalian cells by endocytosis from the cell surface to the endoplasmic reticulum before translocating into the cytosol. Here, SLT was engineered at its N- or C-terminus to carry a peptide derived from influenza virus Matrix protein for delivery to major histocompatibility complex (MHC) class I molecules. We show that SLT N-Ma was capable of sensitising cells for lysis by appropriate cytotoxic T-lymphocytes whilst no killing of SLT-resistant cells was observed. Our results demonstrate that peptide was liberated intracellularly and that retrograde transport of a disarmed cytotoxic protein can intersect the MHC class 1 presentation pathway.
【 授权许可】
Unknown
【 预 览 】
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RO201912020307852ZK.pdf | 175KB | download |