The glucose repressor CRE1 from Sclerotinia sclerotiorum is functionally related to CREA from Aspergillus nidulans but not to the Mig proteins from Saccharomyces cerevisiae

FEBS Letters

Fèvre, Michel¹  Cotton, Pascale¹  Vautard, Géraldine¹ 

[1] Laboratoire de Biologie Cellulaire Fongique, UMR CNRS 5577, INSA, Université Lyon I, 43 bd du 11 Novembre 1918, Bât. 405, 69622 Villeurbanne Cedex, France

关键词: CRE1;    CREA;    Mig1p;    Zinc finger protein;    Glucose repression;    Sclerotinia sclerotiorum;   

DOI  :  10.1016/S0014-5793(99)00691-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We isolated the putative glucose repressor gene cre1 from the phytopathogenic fungus Sclerotinia sclerotiorum. cre1 encodes a 429 amino acid protein 59% similar to the carbon catabolite repressor CREA from Aspergillus nidulans. In addition to the overall amino acid sequence relatedness between CRE1 and CREA proteins, cre1 can functionally complement the A. nidulans creAd30 mutation as assessed by repression of the alcohol dehydrogenase I gene expression. The CRE1 region carrying the two zinc fingers is also very similar to the DNA binding domains of the Saccharomyces cerevisiae glucose repressors Mig1p and Mig2p. Despite the presence in the CRE1 protein of several motifs involved in the regulation of Mig1p activity, cre1 cannot complement mig deficiencies in S. cerevisiae. These data suggest that glucose repression pathways may have evolved differently in yeasts and filamentous fungi.

【 授权许可】

Unknown