Helicobacter pylori lipopolysaccharide enhances the expression of NADPH oxidase components in cultured guinea pig gastric mucosal cells

FEBS Letters

Tsunawaki, Shohko²  Rokutan, Kazuhito¹  Teshima, Shigetada¹ 

[1] Department of Nutrition, School of Medicine, The University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima City, Tokushima 770-8503, Japan;The National Children's Medical Research Center, 3-35-31 Taishido, Setagaya-ku, Tokyo 154-8509, Japan

关键词: NADPH oxidase;    Superoxide anion;    Lipopolysaccharide;    Gastric mucosal cell;    Helicobacter pylori;    LPS;    lipopolysaccharide;    SOD;    superoxide dismutase;   

DOI  :  10.1016/S0014-5793(99)00636-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recently, we showed that cultured guinea pig gastric pit cells possess a phagocyte NADPH oxidase-like activity, which was up-regulated by Helicobacter pylori lipopolysaccharide. We demonstrate here that these cells express all of the phagocyte NADPH oxidase components (gp91-, p22-, p67-, p47-, and p40-phoxes). Treatment with lipopolysaccharide increased the expression of gp91-, p22-, and p67-phoxes, but not that of p47- and p40-phoxes. Intriguingly, the p67-phox expression consistently correlated with up-regulation of superoxide anion-producing ability. Thus, the gastric pit cell NADPH oxidase may play an important role in regulation of the inflammatory response associated with H. pylori infection.

【 授权许可】

Unknown