FEBS Letters | |
Use of a drug‐resistant mutant of stress‐activated protein kinase 2a/p38 to validate the in vivo specificity of SB 203580 | |
van den IJssel, Paul1  Eyers, Patrick A.2  Quinlan, Roy A.1  Cohen, Philip2  Goedert, Michel3  | |
[1] Department of Biochemistry, MSI/WTB Complex, University of Dundee, Dow Street, Dundee DD1 5EH, UK;MRC Protein Phosphorylation Unit, MSI/WTB Complex, University of Dundee, Dow Street, Dundee DD1 5EH, UK;MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK | |
关键词: SAPK2; p38; SB203580; MAPKAP-K2; MSK1; CREB; | |
DOI : 10.1016/S0014-5793(99)00552-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Stress-activated protein kinase 2a, also called p38, is inhibited by SB 203580 and this drug has been used widely to implicate this enzyme in the regulation of many physiological processes. Here, we introduce a novel method of general application, which can be used to establish whether the effects of SB 203580 are mediated via inhibition of stress-activated protein kinase 2a/p38 or whether they result from ‘non-specific’ effects. Four events thought to occur upon activation of stress-activated protein kinase 2a/p38 have been established unequivocally. These are the activation of mitogen-activated protein kinase-activated protein kinase-2 and mitogen- and stress-activated protein kinase-1 and the phosphorylation of their presumed substrates, heat shock protein 27 and the transcription factor cyclic AMP response element binding protein, respectively. In contrast, the SB 203580-induced activation of c-Raf is independent of stress-activated protein kinase 2a/p38 inhibition.
【 授权许可】
Unknown
【 预 览 】
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RO201912020307717ZK.pdf | 238KB | download |