LIS1 and platelet‐activating factor acetylhydrolase (Ib) catalytic subunits, expression in the mouse oocyte and zygote

FEBS Letters

Reiner, Orly¹  Cahana, Aviv¹ 

[1] Department of Molecular Genetics, The Weizmann Institute of Science, 76100 Rehovot, Israel

关键词: LIS1;    Lissencephaly;    Platelet-activating factor;    Platelet-activating factor acetylhydrolase;    Fertilization;   

DOI  :  10.1016/S0014-5793(99)00548-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Platelet-activating factor is a phospholipid with several documented roles in the pre-implantation embryo. Enzymes that belong to the platelet-activating factor acetylhydrolases family inactivate platelet-activating factor. Cytosolic platelet-activating factor acetylhydrolase (Ib) is a heterotetramer composed of two catalytic subunits (α1/α2) and two regulatory LIS1 subunits. The expression of these components was monitored in the mouse oocytes and zygotes using reverse-transcribed PCR and Western blot analysis. Interestingly, these proteins are expressed in the oocyte and zygote and their expression increases after fertilization, probably due to stabilization of maternal RNA. Lis1 mRNA transcription also increases after fertilization. However, assaying for expression of a specific paternal LIS1 isoform detected no zygotic translation in the one cell stage. These findings suggest a potential role for platelet-activating factor acetylhydrolase (Ib) components in the early mouse embryo.

【 授权许可】

Unknown