Defective binding of IRFs to the initiator element of interleukin‐1β‐converting enzyme (ICE) promoter in an interferon‐resistant Daudi subline

FEBS Letters

Nakamura, Mitsuru¹  Furukawa, Yusuke¹  Iwase, Satsuki³  Yamada, Hisashi³  Saito, Shinobu²  Kikuchi, Jiro¹  Nakayama, Ritsuko²  Horiguchi-Yamada, Junko³ 

[1] Division of Molecular Hemopoiesis, Center for Molecular Medicine and Department of Hematology, Jichi Medical School, Tochigi 329-0498, Japan;Department of Genetics, Institute of DNA Medicine, Jikei University School of Medicine, Tokyo 105, Japan;Department of Internal Medicine (Aoto), Jikei University School of Medicine, Tokyo 125-8506, Japan

关键词: Interferon;    Drug resistance;    IRF;    ICE;    Initiator;    Promoter;   

DOI  :  10.1016/S0014-5793(99)00515-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To investigate mechanisms of interferon (IFN) resistance, we have established an IFN-resistant Daudi subline (Daudi^res), which is 1×10⁴ times more resistant to IFN-α than parental cells. Among the IFN-inducible genes examined, only ICE mRNA expression was deficient in Daudi^res cells. We then analyzed the regulatory mechanisms of ICE transcription, and found that IFN-induced activation of the ICE promoter was dependent on the binding of IRFs to its initiator (Inr) element. Inr binding of IRFs was markedly diminished in Daudi^res cells, and forced expression of IRF-1 was able to activate the ICE promoter to the level of parental cells. These results suggest that IRFs and their target genes, as represented by ICE in this study, are involved in IFN resistance.

【 授权许可】

Unknown