Suppression of the neoplastic phenotype by transfection of phospholipase C β 3 to neuroendocrine tumor cells

FEBS Letters

Gobl, Anders¹  Stålberg, Peter¹  Weber, Günther²  Skogseid, Britt¹  Wang, Shu¹  Öberg, Kjell¹  Larsson, Catharina² 

[1] Department of Internal Medicine, University Hospital, S-751 85 Uppsala, Sweden;Department of Molecular Medicine, Clinical Genetics Unit, Karolinska Institute, S-171 76 Stockholm, Sweden

关键词: Phospholipase C β 3;    Neuroendocrine tumor;    Transfection;    Xenograft;    Growth inhibition;    PLCB3 (protein) and PLCB3 (gene);    phosphatidylinositol-specific phospholipase C β 3 (human);    IP3;    inositol 1;    4;    5-trisphosphate;    wt;    wild-type;    vector;    plasmid vector without insert;   

DOI  :  10.1016/S0014-5793(99)00457-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression of phospholipase C β 3 (PLCB3) is low or absent in several neuroendocrine neoplasias. To investigate the role of PLCB3 in the neuroendocrine tumorigenesis, we transfected a PLCB3 construct to three neuroendocrine tumor cell lines with a low PLCB3 expression. The growth rate and tumorigenicity were assessed in vitro by [³H]thymidine incorporation and cell counting, in vivo, by xenografting to nude mice. In vitro, PLCB3 expressing clones showed a significant growth inhibition. The tumor weight was reduced for one of the two xenografted PLCB3-transfected cell lines and in both, a reduced number of proliferating (Ki-67 positive) cells was observed. This study implies an essential role for PLCB3 in the neuroendocrine tumorigenesis.

【 授权许可】

Unknown