Induction of oxidative DNA damage in anaerobes

FEBS Letters

Nakaya, Yasuhiro¹  Kato, Naoki¹  Takeuchi, Toru¹  Morimoto, Kanehisa¹  Watanabe, Kunitomo² 

[1] Department of Hygiene and Preventive Medicine, Osaka University School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan;Institute of Anaerobic Bacteriology, Gifu University School of Medicine, Gifu 500-8705, Japan

关键词: Hydrogen peroxide;    8-hydroxydeoxyguanosine;    Oxidative DNA damage;    Oxygen;    Prevotella melaninogenica;   

DOI  :  10.1016/S0014-5793(99)00497-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We compared oxidative DNA damage in strictly anaerobic Prevotella melaninogenica, aerotolerant anaerobic Bacteroides fragilis, and facultative anaerobic Salmonella typhimurium after exposure to O₂ or H₂O₂. Using HPLC with electrochemical detection, we measured 8-hydroxydeoxyguanosine (8OHdG) as a damage marker. O₂ induced 8OHdG in P. melaninogenica but not in B. fragilis, which shows catalase activity, or in S. typhimurium. In P. melaninogenica, with catalase, O₂ induced less 8OHdG; superoxide dismutase had no effect; with glucose and glucose oxidase, O₂ induced more 8OHdG. H₂O₂ also markedly increased 8OHdG. O₂ was suggested to induce 8OHdG through H₂O₂. O₂ or H₂O₂ decreased survival only in P. melaninogenica. Highly sensitive to oxidative stress, P. melaninogenica could prove useful for investigating oxidative DNA damage.

【 授权许可】

Unknown