H (0) blood group determinant is present on soluble human L‐selectin expressed in BHK‐cells

FEBS Letters

Volz, Barbara²  Tauber, Rudolf²  Mach, Ulrich¹  Reutter, Werner¹  Fieger, Claudia²  Gohlke, Martin¹  Nuck, Rolf¹ 

[1] Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, Arnimallee 22, D-14195 Berlin-Dahlem, Germany;Institut für Klinische Chemie und Pathobiochemie, Universitätsklinikum Benjamin Franklin, Freie Universität, Berlin, Germany

关键词: L-selectin;    Baby hamster kidney cell;    N-Linked glycan;    H (0) blood group determinant;    α1-2FT;    α1-2-fucosyltransferase;    2-AB;    2-aminobenzamide;    BHK;    baby hamster kidney;    CHO Chinese hamster ovary;    HexNAc;    N-acetylhexosamine;    MALDI-TOF-MS;    matrix assisted laser desorption/ionization time of flight mass spectrometry;    PNGase F;    peptide-N 4-(N-acetyl-β-glucosaminyl)asparagine amidase F;    rsL-selectin;    recombinant soluble human L-selectin;   

DOI  :  10.1016/S0014-5793(99)00476-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the present study we show that the H (0) blood group determinant Fucα1-2Galβ1-4GlcNAcβ1-R is present on N-linked glycans of soluble human L-selectin recombinantly expressed in baby hamster kidney (BHK) cells. The glycans were isolated using complementary HPLC techniques and characterized by a combination of exoglycosidase digestion and mass spectrometry. The linkage of the fucose residues was determined by incubation of the glycans with specific fucosidases. The H blood determinant Fucα1-2Galβ1-4GlcNAcβ1 was detected for bi-, 2,4 branched tri- and tetraantennary structures. To our knowledge, the proposed oligosaccharide structures represent a new glycosylation motif for recombinant glycoproteins expressed on BHK cells.

【 授权许可】

Unknown