FEBS Letters | |
H (0) blood group determinant is present on soluble human L‐selectin expressed in BHK‐cells | |
Volz, Barbara2  Tauber, Rudolf2  Mach, Ulrich1  Reutter, Werner1  Fieger, Claudia2  Gohlke, Martin1  Nuck, Rolf1  | |
[1] Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, Arnimallee 22, D-14195 Berlin-Dahlem, Germany;Institut für Klinische Chemie und Pathobiochemie, Universitätsklinikum Benjamin Franklin, Freie Universität, Berlin, Germany | |
关键词: L-selectin; Baby hamster kidney cell; N-Linked glycan; H (0) blood group determinant; α1-2FT; α1-2-fucosyltransferase; 2-AB; 2-aminobenzamide; BHK; baby hamster kidney; CHO Chinese hamster ovary; HexNAc; N-acetylhexosamine; MALDI-TOF-MS; matrix assisted laser desorption/ionization time of flight mass spectrometry; PNGase F; peptide-N 4-(N-acetyl-β-glucosaminyl)asparagine amidase F; rsL-selectin; recombinant soluble human L-selectin; | |
DOI : 10.1016/S0014-5793(99)00476-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In the present study we show that the H (0) blood group determinant Fucα1-2Galβ1-4GlcNAcβ1-R is present on N-linked glycans of soluble human L-selectin recombinantly expressed in baby hamster kidney (BHK) cells. The glycans were isolated using complementary HPLC techniques and characterized by a combination of exoglycosidase digestion and mass spectrometry. The linkage of the fucose residues was determined by incubation of the glycans with specific fucosidases. The H blood determinant Fucα1-2Galβ1-4GlcNAcβ1 was detected for bi-, 2,4 branched tri- and tetraantennary structures. To our knowledge, the proposed oligosaccharide structures represent a new glycosylation motif for recombinant glycoproteins expressed on BHK cells.
【 授权许可】
Unknown
【 预 览 】
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RO201912020307643ZK.pdf | 183KB | download |