Dual specificity antibodies using a double‐stranded oligonucleotide bridge

FEBS Letters

Chaudri, Zahida N.²  Roitt, Ivan M.²  Panayotou, George³  Klonisch, Thomas²  Lund, Torben²  Delves, Peter J.²  Bartlet-Jones, Michael¹ 

[1] Imperial Cancer Research Fund, London, UK;Department of Immunology, University College London, The Windeyer Institute for Medical Sciences, 46 Cleveland Street, London W1P 6DB, UK;Ludwig Institute for Cancer Research, London, UK

关键词: Bispecific;    Antibody;    Oligonucleotide;    BIAcore;    Epitope;    Linker;   

DOI  :  10.1016/S0014-5793(99)00466-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The covalent conjugation of oligonucleotides to antibody Fab’ fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of a second specificity. The antibodies were then allowed to hybridize by base pairing of the complementary nucleotide sequences and the generation of bispecific antibody was analyzed on SDS-PAGE and confirmed using BIAcore analysis. The strategy of complementary oligonucleotide-linked bispecific molecules is not limited to antibodies but is applicable to linking any two molecules of different characteristics.

【 授权许可】

Unknown