Thiamine repression and pyruvate decarboxylase autoregulation independently control the expression of the Saccharomyces cerevisiae PDC5 gene

FEBS Letters

Karlsson, Karl-Anders⁴  Richards, Emma J.²  Hohmann, Stefan¹  Pretorius, Gert H.J.³  Norbeck, Joakim¹  Byrne, Kerry L.²  Muller, Elize H.¹  Meacock, Peter A.²  Blomberg, Anders¹ 

[1] Department of Cell and Molecular Biology/Microbiology, Göteborg University, Box 462, S-405 30 Göteborg, Sweden;Department of Genetics, University of Leicester, Leicester, UK;Department of Microbiology and Biochemistry, University of the Orange Free State, Bloemfontein, South Africa;Department of Medical Biochemistry, Göteborg University, Göteborg, Sweden

关键词: Thiamine;    Pyruvate decarboxylase;    Autoregulation;    Gene expression;    Yeast;    Pdc;    pyruvate decarboxylase;    PDC;    genes encoding Pdc or regulators thereof;    ThDP;    thiamine diphosphate;    THI;    genes encoding enzymes or regulators of ThDP metabolism;    2D-PAGE;    two-dimensional polyacrylamide gel electrophoresis;    MOWES;    molecular weight search;   

DOI  :  10.1016/S0014-5793(99)00449-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Saccharomyces cerevisiae gene PDC5 encodes the minor isoform of pyruvate decarboxylase (Pdc). In this work we show that expression of PDC5 but not that of PDC1, which encodes the major isoform, is repressed by thiamine. Hence, under thiamine limitation both PDC1 and PDC5 are expressed. PDC5 also becomes strongly expressed in a pdc1Δ mutant. Two-dimensional gel electrophoresis of whole protein extracts shows that thiamine limitation stimulates the production of THI gene products and of Pdc5p. Deletion of PDC1 only stimulates production of Pdc5p. We conclude that the stimulation of PDC5 expression in a pdc1Δ mutant is not due to a response to thiamine limitation.

【 授权许可】

Unknown