Constitutive and nitrogen‐regulated promoters of the petH gene encoding ferredoxin:NADP+ reductase in the heterocyst‐forming cyanobacterium Anabaena sp

FEBS Letters

Flores, Enrique²  Valladares, Ana²  Herrero, Antonia²  Fillat, María F.¹  Muro-Pastor, Alicia M.² 

[1] Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, E-50009 Zaragoza, Spain;Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-Universidad de Sevilla, Centro de Investigaciones Científicas Isla de la Cartuja, Avda. Américo Vespucio s/n, E-41092 Sevilla, Spain

关键词: Ferredoxin:NADP+ reductase;    nifH;    NtcA;    petH;    Nitrogen fixation;    Anabaena sp;   

DOI  :  10.1016/S0014-5793(99)00404-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Determination of the putative transcription start points of the petH gene encoding ferredoxin:NADP⁺ reductase in the heterocyst-forming cyanobacteria Anabaena sp. PCC 7119 and PCC 7120 showed that this gene is transcribed from two promoters, one constitutively used under different conditions of nitrogen nutrition and the other one used in cells subjected to nitrogen stepdown and in nitrogen-fixing filaments. The latter promoter, whose use was NtcA-dependent but HetR-independent, was functional in heterocysts. The N-control transcriptional regulator NtcA was observed to bind in vitro to this promoter. For the sake of comparison, the transcription start points of the nifHDK operon in strain PCC 7120 and binding of NtcA to the nifHDK promoter were also examined.

【 授权许可】

Unknown