Cooperative interaction of NF‐κB and C/EBP binding sites is necessary for manganese superoxide dismutase gene transcription mediated by lipopolysaccharide and interferon‐γ

FEBS Letters

Xiao, Hengyi¹  Takeuchi, Akihide¹  Hasegawa, Tadao¹  Maehara, Kayoko¹  Isobe, Ken-ichi¹  Abe, Ryoichi¹ 

[1] Department of Basic Gerontology, National Institute for Longevity Sciences, 36-3 Gengo, Morioka-cho, Obu, Aichi 474-8522, Japan

关键词: Manganese superoxide dismutase;    Nuclear factor-κB;    CCAAT/enhancer binding protein;    Lipopolysaccharide;    Mn-SOD;    manganese superoxide dismutase;    LPS;    lipopolysaccharide;    IFN-γ;    interferon-γ;    TNF-α;    tumor necrosis factor-α;    TNFRE;    TNF-responsive element;    IL;    interleukin;    NF-κB;    nuclear factor-κB;    C/EBP;    CCAAT/enhancer binding protein;    EMSA;    electrophoretic mobility shift assay;    WT;    wild type;   

DOI  :  10.1016/S0014-5793(99)00408-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of the manganese superoxide dismutase (Mn-SOD) is induced by pro-inflammatory cytokines. We investigated the cis-acting elements within a tumor necrosis factor-responsive element (TNFRE) which was identified in the second intron of the murine Mn-SOD gene. Site-directed mutagenesis, reporter plasmid transfection studies and electrophoretic mobility shift assays demonstrated that inducible transcription factors enhanced the transcriptional activity of the Mn-SOD gene through the TNFRE. The cooperation between proteins binding to the newly identified NF-κB and C/EBP sites led to synergistic gene transcription. This report provides the first evidence that cooperation between two distinct cis-acting elements may be required for induction of Mn-SOD gene expression mediated by lipopolysaccharide and interferon-γ.

【 授权许可】

Unknown