Differential potency of two crosslinking plant lectins to induce formation of haptenic‐sugar‐resistant aggregates of rat thymocytes by post‐binding signaling

FEBS Letters

Gabius, H.-J.²  Gorudko, I.V.¹  Timoshenko, A.V.¹  Cherenkevich, S.N.¹ 

[1] Department of Biophysics, Belarusian State University, Pr. F. Skaryny 4, Minsk 220050, Belarus;Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University, Veterinärstr. 13, D-80539 Munich, Germany

关键词: Agglutinin;    Aggregation;    Cell adhesion;    Lectin;    Thymocyte;    Trans-membrane signaling;    Con A;    concanavalin A;    D609;    tricyclodecan-9-yl-xanthogenate potassium salt;    GlcNAc;    N-acetyl-d-glucosamine;    HSR;    haptenic-sugar-resistant;    α-MM;    α-methyl-d-mannopyranoside;    NDGA;    nordihydroguaiaretic acid;    NEM;    N-ethylmaleimide;    PBS;    phosphate-buffered saline;    STA;    Solanum tuberosum agglutinin;    TFP;    trifluoperazine;   

DOI  :  10.1016/S0014-5793(99)00329-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To evaluate the significance of post-binding events for stable aggregate formation, the aggregation/dissociation of rat thymocytes initiated by two crosslinking plant lectins, namely concanavalin A (Con A) and Solanum tuberosum agglutinin (STA), were comparatively studied. Despite intimate cell contacts in the aggregates only Con A led to establishment of haptenic-sugar-resistant (HSR) complexes. The presence of inhibitor II of diacylglycerol kinase, a dual calmodulin antagonist/protein kinase C inhibitor (trifluoperazine), and a sulfhydryl group reagent (N-ethylmaleimide) impaired this process. The obtained results indicate that the formation of HSR cellular contacts is not an automatic response to lectin-dependent cell association. In contrast to STA, Con A binding elicits this reaction with involvement of diacylglycerol kinase, protein kinase C and/or calmodulin as well as thiol level perturbation, as inferred by the application of target-selective inhibitors.

【 授权许可】

Unknown