FEBS Letters | |
Activation pattern of mitogen‐activated protein kinases elicited by peroxynitrite: attenuation by selenite supplementation | |
Sies, Helmut1  Klotz, Lars-Oliver1  Schieke, Stefan M1  Briviba, Karlis1  | |
[1] Institut für Physiologische Chemie I, Heinrich-Heine-Universität, Postfach 101007, D-40001 Düsseldorf, Germany | |
关键词: Peroxynitrite; Signal transduction; p38; JNK1/2; ERK1/2; Selenoprotein; | |
DOI : 10.1016/S0014-5793(99)00372-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Peroxynitrite is a mediator of toxicity in pathological processes in vivo and causes damage by oxidation and nitration reactions. Here, we report a differential induction of mitogen-activated protein kinases (MAPKs) in WB-F344 rat liver epithelial cells by peroxynitrite. For the exposure of cultured cells with peroxynitrite, we employed a newly developed infusion method. At 6.5 μM steady-state concentration, the activation of p38 MAPK was immediate, while JNK1/2 and ERK1/2 were activated 60 min and 15 min subsequent to 3 min of exposure to peroxynitrite, respectively. Protein-bound 3-nitrotyrosine was detected. When cells were grown in a medium supplemented with sodium selenite (1 μM) for 48 h, complete protection was afforded against the activation of p38 and against nitration of tyrosine residues. These data suggest a new role for peroxynitrite in activating signal transduction pathways capable of modulating gene expression. Further, the abolition of the effects of peroxynitrite by selenite supplementation suggests a protective role of selenium-containing proteins.
【 授权许可】
Unknown
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