Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin‐activating enzyme complex

FEBS Letters

Yeh, Edward T.H.¹  Gong, Limin¹  Millas, Stefanos¹  Li, Bing¹ 

[1] Research Center for Cardiovascular Diseases, Institute of Molecular Medicine for the Prevention of Human Diseases, University of Texas-Houston Health Science Center, Houston, TX 77030, USA

关键词: Ubiquitin;    Sentrin;    SUMO;    UBC9;    UBA2;    AOS1;   

DOI  :  10.1016/S0014-5793(99)00367-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sentrin-1/SUMO-1 is a novel ubiquitin-like protein, which can covalently modify a limited number of cellular proteins. Here we report the identification of the sentrin-activating enzyme complex, which consists of two proteins AOS1 and UBA2. Human AOS1 is homologous to the N-terminal half of E1, whereas human UBA2 is homologous to the C-terminal half of E1. The human UBA2 gene is located on chromosome 19q12. Human UBA2 could form a β-mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8, in the presence of AOS1. Identification of human UBA2 and AOS1 should allow a more detailed analysis of the enzymology of the activation of ubiquitin-like proteins.

【 授权许可】

Unknown