Pseudoazurin mediates periplasmic electron flow in a mutant strain of Paracoccus denitrificans lacking cytochrome c550

FEBS Letters

Van Spanning, Rob J.M.³  Tesařı́k, Radek²  Kučera, Igor¹  Koutný, Marek¹  Turánek, Jaroslav² 

[1] Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, CZ-61137 Brno, Czech Republic;Department of Immunology, Veterinary Research Institute, Hudcova 70, CZ-62132 Brno, Czech Republic;Department of Molecular Cell Physiology, Faculty of Biology, Free University, De Boelelaan 1087, NL-1081 HV Amsterdam, The Netherlands

关键词: Pseudoazurin;    Nitrite reduction;    Electron transport;    Paracoccus denitrificans;    AAS;    atomic absorption spectroscopy;    DDC;    diethyldithiocarbamate;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(99)00345-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A periplasmic protein able to transfer electrons from cytoplasmic membrane to the periplasmic nitrite reductase (cytochrome cd₁ ) has been purified from the anoxically grown cytochrome c₅₅₀ mutant strain Pd2121 and shown to be pseudoazurin by several independent criteria (molecular mass, copper content, visible spectrum, N-terminal amino acid sequence). Under our assay conditions, the half-saturation of electron transport occurred at about 10 μM pseudoazurin; the reaction was retarded by increasing ionic strength.

【 授权许可】

Unknown