Novel BNIP1 variants and their interaction with BCL2 family members

FEBS Letters

Meyhack, Bernd¹  Heim, Jutta¹  Zhang, Hong¹ 

[1] Novartis Pharma AG, Oncology, Molecular Genetics, CH-4002 Basel, Switzerland

关键词: BNIP1;    Variant;    BCL2;    BCL2L1;    BAX;    Interaction;    PCR;    polymerase chain reaction;    EST;    expressed sequence tag;    STS;    sequence tagged site;    PAGE;    polyacrylamide gel electrophoresis;    CAT;    chloramphenicol acetyltransferase;    GST;    glutathione S-transferase;    BH3;    BCL2 homology region 3;    BNIP1;    BCL2/adenovirus E1B 19 kDa interacting protein 1 (previously Nip1);    BNIP1vs;    BNIP1 splice variants;    including BNIP1-a;    BNIP1-b and BNIP1-c (previously: Nip1s1;    Nip1s2 and Nip1s3);    BCL2A1;    BCL2-related protein A1 (previously Bfl-1);    BCL2L1;    BCL2-like 1 (previously Bcl-xL);   

DOI  :  10.1016/S0014-5793(99)00335-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By PCR and EST database searches we have identified three novel BNIP1 splice variants, and found that one of them, BNIP1-b, contains a highly conserved BH3 domain. The BNIP1 gene has been assigned to chromosome 5q33–34. Using in vitro protein-protein interaction assays, all BNIP1 variants were shown to interact with BCL2 and also with BCL2L1 (previously Bcl-xL). These interactions are BH3-independent. Furthermore, the BNIP1 variants cannot interact with BAX. The results suggest that the BNIP1 variants are novel members of the BCL2 family but function through a mechanism different from other BH3-only members.

【 授权许可】

Unknown