Negative cooperativity between juxtaposed E‐box and cAMP/TPA responsive elements in the cholecystokinin gene promoter

FEBS Letters

Rourke, Ian J²  Nerlov, Claus¹  Rehfeld, Jens F²  Nielsen, Finn C²  Hansen, Thomas vO² 

[1] The Laboratory for Gene Therapy Research, Rigshospitalet, Copenhagen, Denmark;Department of Clinical Biochemistry, Rigshospitalet, Blegdamsvej 9, DK-2100 Copenhagen Ø, Denmark

关键词: Cholecystokinin (CCK) gene promoter;    Juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE);    Negative cooperativity;   

DOI  :  10.1016/S0014-5793(99)00320-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/TRE. Mutation of the E-box enhanced the activation by c-Jun/c-Fos, as well as stimulation by forskolin and bFGF, that acts through the CRE/TRE site. Moreover, c-Jun/c-Fos stimulation was inhibited by co-expression of c-Myc and Max. The results indicate that factors associating with the E-box exhibit a negative cooperative effect on the activation via the CRE/TRE element. We propose that this mechanism plays a significant role in CCK gene transcription and other genes with juxtaposed E-box and CRE/TRE.

【 授权许可】

Unknown