Structural requirement of leucine for activation of p70 S6 kinase

FEBS Letters

Miyake, Hiroshi¹  Tanaka, Noriaki³  Shigemitsu, Kaori²  Hara, Kenta²  Yonezawa, Kazuyoshi²  Tsujishita, Yosuke²  Hidayat, Sujuti² 

[1] Fujisawa Pharmaceutical Co., Ltd., 3-4-7 Doshomachi, Chuo-ku, Osaka 541-8514, Japan;Biosignal Research Center, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan;The First Department of Surgery, Okayama University School of Medicine, 2-5-1 Shikata-cho, Okayama 700-0914, Japan

关键词: Leucine;    p70 S6 kinase;    Amino acid;    Translational control;    CHO-IR;    Chinese hamster ovary cells overexpressing human insulin receptors;    DMEM;    Dulbecco's modified Eagle's medium;    αMEM;    minimal essential medium α;    MOPS;    4-morpholinepropanesulfonic acid;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(99)00304-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The addition of leucine induced activation of p70^S6k in amino acid-depleted H4IIE cells. Whereas the activation of p70^S6k by leucine was transient, the complete amino acid stimulated p70^S6k more persistently. The effect of leucine on p70^S6k was sensitive to rapamycin, but less sensitive to wortmannin. Using various amino acids and derivatives of leucine, we found that the chirality, the structure of the four branched hydrocarbons, and the primary amine are required for the ability of leucine to stimulate p70^S6k, indicating that the structural requirement of leucine to induce p70^S6k activation is very strict and precise. In addition, some leucine derivatives exhibited the ability to stimulate p70^S6k and the other derivatives acted as inhibitors against the leucine-induced activation of p70^S6k.

【 授权许可】

Unknown