A single uridine modification at the wobble position of an artificial tRNA enhances wobbling in an Escherichia coli cell‐free translation system

FEBS Letters

Okumura, Shuhei¹  Takai, Kazuyuki¹  Takaku, Hiroshi¹  Yokoyama, Shigeyuki²  Hosono, Kazumi¹ 

[1] Department of Industrial Chemistry, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan;Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

关键词: 5-Methoxyuridine;    Codon recognition;    Post-transcriptional modification;    Cell-free protein synthesis;    tRNA selection;    Escherichia coli;   

DOI  :  10.1016/S0014-5793(99)00255-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

5-Methoxyuridine was introduced into the first position of the anticodon of the unmodified form of tRNA₁ ^Ser from Escherichia coli. The codon reading efficiencies of this tRNA (tRNA(5-methoxyuridine UGA)) relative to those of the unmodified counterpart (tRNA(UGA)) were measured in a cell-free translation system. tRNA(5-methoxyuridine UGA) was more efficient than tRNA(UGA) in the reading of the UCU and UCG codons and was less efficient in the reading of the UCA codon. Thus, the single modification of U to 5-methoxyuridine can enhance the wobble readings.

【 授权许可】

Unknown