期刊论文详细信息
FEBS Letters
Unique topology of the internal repeats in the cardiac Na+/Ca2+ exchanger
Nakamura, Tomoe Y2  Uehara, Akira1  Iwamoto, Takahiro2  Imanaga, Issei1  Shigekawa, Munekazu2  Pan, Yan2 
[1] Department of Physiology, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan;Department of Molecular Physiology, National Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan
关键词: Na+/Ca2+ exchanger;    Membrane topology;    Cysteine susceptibility analysis;    Epitope tagging;   
DOI  :  10.1016/S0014-5793(99)00218-5
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Hydropathy analysis predicts 11 transmembrane helices in the cardiac Na+/Ca2+ exchanger. Using cysteine susceptibility analysis and epitope tagging, we here studied the membrane topology of the exchanger, in particular of the highly conserved internal α-1 and α-2 repeats. Unexpectedly, we found that the connecting loop in the α-1 repeat forms a re-entrant membrane loop with both ends facing the extracellular side and one residue (Asn-125) being accessible from the inside and that the region containing the α-2 repeat is mostly accessible from the cytoplasm. Together with other data, we propose that the exchanger may consist of nine transmembrane helices.

【 授权许可】

Unknown   

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