FEBS Letters | |
The assumption that nitric oxide inhibits mitochondrial ATP synthesis is correct | |
Brookes, Paul S.2  Bolaños, Juan P.1  Heales, Simon J.R.3  | |
[1] Departamento de Bioquı́mica y Biologı́a Molecular, Universidad de Salamanca, Edificio Departamental, Avda. del Campo Charro, s/n 37007 Salamanca, Spain;Department of Pathology, University of Alabama at Birmingham, 1670 University Blvd., Birmingham, AL 35294, USA;Departments of Neurochemistry and Clinical Biochemistry, Institute of Neurology and National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N 3BG, UK | |
关键词: Brain mitochondria; Nitric oxide; ATP; Oxidative stress; Neurodegeneration; | |
DOI : 10.1016/S0014-5793(99)00217-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The assumption that reversible inhibition of mitochondrial respiration by nitric oxide (NO⋅) represents inhibition of ATP synthesis is unproven. NO⋅ could theoretically inhibit the oxygen consumption with continued ATP synthesis, by acting as an electron acceptor from cytochrome c or as a terminal electron acceptor in stead of oxygen. We report here that NO⋅ does reversibly inhibit brain mitochondrial ATP synthesis with a time course similar to its inhibition of respiration. Whilst such inhibition was largely reversible, there appeared to be a small irreversible component which may theoretically be due to peroxynitrite formation, i.e. as a result of the reaction between NO⋅ and superoxide, generated by the mitochondrial respiratory chain.
【 授权许可】
Unknown
【 预 览 】
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RO201912020307403ZK.pdf | 70KB | download |