The assumption that nitric oxide inhibits mitochondrial ATP synthesis is correct

FEBS Letters

Brookes, Paul S.²  Bolaños, Juan P.¹  Heales, Simon J.R.³ 

[1] Departamento de Bioquı́mica y Biologı́a Molecular, Universidad de Salamanca, Edificio Departamental, Avda. del Campo Charro, s/n 37007 Salamanca, Spain;Department of Pathology, University of Alabama at Birmingham, 1670 University Blvd., Birmingham, AL 35294, USA;Departments of Neurochemistry and Clinical Biochemistry, Institute of Neurology and National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N 3BG, UK

关键词: Brain mitochondria;    Nitric oxide;    ATP;    Oxidative stress;    Neurodegeneration;   

DOI  :  10.1016/S0014-5793(99)00217-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The assumption that reversible inhibition of mitochondrial respiration by nitric oxide (NO^⋅) represents inhibition of ATP synthesis is unproven. NO^⋅ could theoretically inhibit the oxygen consumption with continued ATP synthesis, by acting as an electron acceptor from cytochrome c or as a terminal electron acceptor in stead of oxygen. We report here that NO^⋅ does reversibly inhibit brain mitochondrial ATP synthesis with a time course similar to its inhibition of respiration. Whilst such inhibition was largely reversible, there appeared to be a small irreversible component which may theoretically be due to peroxynitrite formation, i.e. as a result of the reaction between NO^⋅ and superoxide, generated by the mitochondrial respiratory chain.

【 授权许可】

Unknown