Alpha4 protein as a common regulator of type 2A‐related serine/threonine protein phosphatases 1

FEBS Letters

Nanahoshi, Masakazu¹  Sakaguchi, Nobuo²  Tokunaga, Chiharu¹  Hara, Kenta¹  Yonezawa, Kazuyoshi¹  Tsujishita, Yosuke¹  Inui, Seiji² 

[1] Biosignal Research Center, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan;Department of Immunology, Kumamoto University School of Medicine, 2-2-1 Honjo, Kumamoto 860-0811, Japan

关键词: Alpha4;    Protein phosphatase 2A;    Protein phosphatase 4;    Protein phosphatase 6;    PR65;    PP2A;    protein phosphatase 2A;    PP4;    protein phosphatase 4;    PP6;    protein phosphatase 6;    pNPP;    p-nitrophenyl phosphoric acid;    TOR;    target of rapamycin;   

DOI  :  10.1016/S0014-5793(99)00189-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The catalytic activity of the C subunit of serine/threonine phosphatase 2A is regulated by the association with A (PR65) and B subunits. It has been reported that the alpha4 protein, a yeast homolog of the Tap42 protein, binds the C subunit of serine/threonine phosphatase 2A and protein phosphatase 2A-related protein phosphatases such as protein phosphatase 4 and protein phosphatase 6. In the present study, we showed that alpha4 binds these three phosphatases and the association of alpha4 reduces the activities of these phosphatases in vitro. In contrast, PR65 binds to the C subunit of serine/threonine phosphatase 2A but not to protein phosphatase 4 and protein phosphatase 6. These results suggest that the alpha4 protein is a common regulator of the C subunit of serine/threonine phosphatase 2A and protein phosphatase 2A-related protein phosphatases.

【 授权许可】

Unknown