Stoichiometry of dipalmitoylphosphatidylcholine‐DNA interaction in the presence of Ca2+: a temperature‐scanning ultrasonic study

FEBS Letters

Kharakoz, D.P.¹  Gorelov, A.V.¹  Dawson, K.A.²  Khusainova, R.S.¹ 

[1] Institute of Theoretical and Experimental Biophysics, 142 292 Pushchino, Russia;Irish Centre for Colloid Science and Biomaterials, Chemistry Department, University College Dublin, Belfield, Dublin 4, Ireland

关键词: DNA-lipid interaction;    Phase transition;    Transfection;    Ultrasonics;    DPPC;    dipalmitoylphosphatidylcholine;    MLV;    multilamellar vesicle;    ULV;    unilamellar vesicle;    HEPES;    N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid];   

DOI  :  10.1016/S0014-5793(99)00165-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA-DPPC complexes can be prepared by means of a single step procedure of mixing DNA solution and aqueous lipid dispersion in the presence of calcium ions. Interaction between DPPC and DNA brings about a biphasic shape of melting curves corresponding to the free lipid and the strongly bound one. The amount of the strongly bound lipid is 5 molecules per nucleotide which is close to the size of the first lipid monolayer around DNA molecule.

【 授权许可】

Unknown