Actin dynamics in lamellipodia of migrating border cells in the Drosophila ovary revealed by a GFP‐actin fusion protein

FEBS Letters

Tsukita, Shoichiro¹  Verkhusha, Vladislav V¹  Oda, Hiroki¹ 

[1] Tsukita Cell Axis Project, ERATO, JST, Kyoto Research Park, Minami-machi, Chudoji, Shimogyo-ku, Kyoto 600-8813, Japan

关键词: Green fluorescent protein-actin fusion;    Border cell migration;    Actin dynamics;    Forepart lamellipodium;    BC;    border cell;    NC;    nurse cell;    GFP;    green fluorescent protein;    BOC;    bundle-organizing center;    BDM;    2;    3-butanedione monoxime;   

DOI  :  10.1016/S0014-5793(99)00124-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Directional migration of border cells in the Drosophila egg chambers is a developmentally regulated event that requires dynamic cellular functions. In this study, the electron microscopic observation of migrating border cells revealed loose actin bundles in forepart lamellipodia and numerous microvilli extending from nurse cells and providing multiple adhesive contacts with border cells. To analyze the dynamics of actin in migrating border cells in vivo, we constructed a green fluorescent protein-actin fusion protein and induced its expression in Drosophila using the GAL4/UAS system. The green fluorescent protein-actin was incorporated into the actin bundles and it enabled visualization of the rapid cytoskeletal changes in border cell lamellipodia. During the growth of the lamellipodia, the actin bundles that increased in number and size radiated from the bundle-organizing center. Quantification of the fluorescence intensity showed that an accumulation of bundle-associated and spotted green fluorescent protein-actin signals took place during their centripetal movement. Our results favored a treadmilling model for actin behavior in border cell lamellipodia.

【 授权许可】

Unknown